MORPHOLOGICAL CHANGES OF HUMAN MESENCHYMAL STEM CELLS UNDER 3D CLINOSTAT CULTUREHO NGUYEN QUYNH CHI AND HOANG NGHIA SON
This study aimed to estimate morphological changes of human mesenchymal stem cells (hMSCs) under 3D clinostat culture which generated simulated microgravity (SMG) condition. hMSCs were induced simulated microgravity (<10-3 G) for 3 days, while control group was conducted in the normal G condition (1G). The results showed that the cellular area of hMSCs from SMG group was higher than control group (14083.34 Â± 1069.38 Î¼m2 vs. 11368.67 Â± 535.27 Î¼m2, respectively). The FSC value of hMSCs from SMG group was higher than control group (10803988.58 Â± 20960.12 vs. 9829898.02 Â± 206370.30, respectively). Thus, SMG condition induced the increase in the size of hMSCs. Microtubule staining analysis demonstrated that the intensity of the microtubule of hMSCs from control group was higher than SMG group (3980694.27 Â± 842699.73 vs. 2860286.60 Â± 338612.60, respectively). Moreover, the intensity of the microfilament of hMSCs from control group was higher than SMG group (4276497.43 Â± 1190673.58 vs. 3364019.71 Â± 688901.20, respectively). In addition, SMG condition moderated hMSC nuclear morphology, in which the transformation of round-shape to oval-shape was observed in hMSCs induced by SMG. These results revealed that SMG condition modified the cytoskeleton distribution in hMSCs, which leads to the morphological changes.