Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 22, Issue 3, 2020; Page No.(395-404)

OPTIMAL CONDITIONS, EXTRACTION, AND PARTIAL PURIFICATION OF A POTENTIAL NOVEL ANTIMICROBIAL COMPOUND PRODUCED BY STREPTOMYCES N-404 STRAIN

ISMAIL SAADOUN, FOUAD AL-MOMANI AND MAHER OBEIDAT

Abstract

In this study the optimal conditions in addition to extraction, partial purification and characterization of antimicrobial compound produced by Streptomyces N-404 strain were determined. Results showed that Streptomyces N-404 strain optimally produced the antimicrobial compound against the test organisms after 5 days of incubation in nutrient broth at 28 ºC and initial pH of 8.5. Analysis of the cell free broth revealed that it contains an antimicrobial compound which was stable over a wide range of pHs and temperatures, and retained inhibitory activity after heating and treatment with different enzymes. The minimum inhibitory concentration (MIC) of the water soluble of concentrated ethanolic crude extract against Staphylococcus aureus and Escherichia coli was 5 and 10 mg/mL, respectively. Purification experiments for the active compound out of permutite column by a gradient solution of KOH revealed the elution of one major peak and a 74% increase in activity against S. aureus with a maximum inhibition zone diameter of 33 mm. When Streptomyces N-404 strain was tested for its sensitivity to gentamycin and kanamycin and the partial purified active compound was compared to these antibiotics, data indicated that this strain is not producing these 2 antibiotics, and the active compound resemble them in their temperature and pH stability, but differs from these compounds in TLC pattern, adsorption to charcoal under basic and acidic conditions, and UV spectrum. These findings stress the novelty of the inhibitory compound produced by Streptomyces N-404 strain.

Journal Issues
Vol 22, Issue 3, 2020
Vol 22, Issue 2, 2020
Vol 22, Issue 1, 2020
Vol 21, Issue 4, 2019
Vol 21, Nov. Suppl. Issue, 2019
Vol 21, Issue 3, 2019
Vol 21, Issue 2, 2019
Vol 21, Issue 1, 2019
Vol 20, Dec. Suppl. No.2, 2018
Vol 20, Issue 4, 2018
Vol 20, Dec. Suppl. Issue, 2018
Vol 20, Oct Suppl. Issue, 2018
Vol 20, Issue 3, 2018
Vol 20, Issue 2, 2018
Vol 20, Issue 1, 2018
Vol 20, Feb. Suppl. Issue, 2018
Vol 19, Issue 4, 2017
Vol 19, Nov. Suppl, Issue 2017
Vol 19, Issue 3, 2017
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!