BIOSURFACTANTS PRODUCTION USING BACTERIAL ISOLATE FROM CANGAR HOT SPRING, EAST JAVA, INDONESIAAlmando Geraldi, Aulia Azzahra, Lillah Asritafriha, Rizki Amaliah Zain, Us Watun Nurul Khasanah, Nastiti Trikurniadewi and Ni’matuzahrohAbstract Biosurfactants, a group of surface-active agents mainly produced by microorganisms, are one of the most sought after high-value bioproducts due to their multi functionality in different industrial processes. Furthermore, biosurfactants are regarded as more economical to produce, sustainable, and environment friendly than petroleum-based synthetic surfactants. At the industrial level, thermostability of biosurfactants would give an advantage in many of their applications. In this research, we explore the microorganism biodiversity of Cangar Hot Spring in East Java, Indonesia to isolate biosurfactant-producing bacteria. One bacteria which identified as Bacillus subtilis subsp. Inaquosorum CGR-1 strain produced biosurfactants at 40- 50 oC. Furthermore, crude biosurfactants produced by this strain could reduce surface tension and showed emulsification activity. The biosurfactant will be further characterized and utilized for different processes which occur in high temperature. |