BIO REMEDIATION OF TEXTILE EFFLUENT USING BACTERIAL CONSORTIUM OBTAINED FROM INDUSTRIALLY POLLUTED SITEPoonam Ranga, Deepansh Sharma and Baljeet Singh SaharanAbstract The inappropriate and unselective dumping of textile effluent in natural waters and land is creating serious complications and it has also antagonistic effects on various crops. The effect of various combinations of selected isolates on textile effluent decolorization was assessed, to develop an effective consortium. The consortium of isolate SPR28 and SPR42 (1:1) displayed maximal decolorization of 42.5% after 84 h of incubation. The consortium of isolate, SPR28 and SPR48 (1:1) displayed maximum decolorization of 39.8% after 96 h of incubation. Molecular characterization of the 16S rRNA sequences showed similarity of isolate SPR42 with Bacillus subtilis and isolate SPR48 to Bacillus cereus. Toxicity test of bioremediated effluent was performed at 100% conc. of effluent. Untreated effluent showed 30.3% seed germination and 3.03 germination speed. On the other hand, seed exposed with bioremediated effluent lead to% germination and germination speed of 98.7% and 10.8, respectively, for consortium of SPR42 and SPR48. However, seed treated with P. putida bioremediated effluent showed 70.9% germination with a germination speed of 5.16. Microbial decolourization holds potential and would be used to advance a low cost, biodegradable microbial technology for the removal of textile dyes/effluent. |