Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 22, Issue 2, 2020; Page No.(386-390)

ANTIBACTERIAL ACTIVITY OF HAMELIA PATENS AGAINST HUMAN PATHOGENS

ASHWATHY G., ALLY JAMES AND KRISHNAKUMAR K.

Abstract

Hamelia patens Jacq. (Rubiaceae) is a common ornamental plant with many medicinal properties. The present investigation was carried out in order to assess the antibacterial property of the ethanolic and aqueous extracts of H. patens Jacq. on the common human pathogens viz. Escherichia coli, Vibrio harveyi, Staphylococcus aureus and Bacillus cereus by Kirby- Bauer disc diffusion assay. The antibacterial activity of the biosynthesized silver nanoparticles was also carried out. The preliminary phytochemical screening revealed the presence of alkaloids, tannins, phenols, glycosides, saponins, flavonoids, protein, carbohydrate and terpenoids in both aqueous and ethanolic extracts. The nanoparticles biosynthesized using H. patens were spherical in shape and grayish in color. Both the extracts showed similar activities against all four strains of bacteria. Nanoparticle solution showed considerable activity against Escherichia coli with an inhibition zone of 11mm. Maximum zone of inhibition was exhibited by the standard antibiotic drug Amoxycillin.

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