BIOREMEDIATION OF AZO DYE AND TEXTILE EFFLUENTS USING PSEUDOMONAS PUTIDA MTCC 2445POONAM RANGA, DEEPANSH SHARMA AND BALJEET SINGH SAHARANAbstract Due to industrialization in the last century, lots of chemical including dyes are manufactured and used in various industrial process. In India, out of total export about 1/3 is from textile industries and largely accounts for aquatic and soil pollution due to the presence of the various dyes. Approximately 2500 small or medium scale or large-scale textile industries are scattered all over the country in different clusters. In the present study Pseudomonas putida MTCC 2445 has been selected based on its ability to degrade the textile dyes in primary screening. Strain MTCC 2445 was found to degrade Yellow HEGR dye was observed after 84 h of incubation. The maximum decolorization at concentrations of 50 mgL-1 was 68.7%. In direct textile industry effluent, P. putida was able to decolorize 25 and 50 % textile effluent concentration after 12 h of incubation time. The effect of different temperatures showed that maximum decolorization was at 37 °C after 84 h of incubation. The maximum decolorization of Yellow HEGR dye (45.8 %) and textile effluent (31.0 %) was observed at pH 8.0 after 84 h and 96 h of incubation, respectively. The effect of aeration was studied on the decolorization of dyes and effluents. It was observed that the static conditions showed better decolorization than shaking at 200 rpm. The non-living cells showed no decolorization of dyes or effluent confirming biodegradation of dyes and effluent. It was found during phytotoxicity assessment that, biodegraded textile effluent is less toxic as compared with the non-treated effluent. P. putida was found an appropriate strain to reduce the dye load in media broth and textile effluent under laboratory conditions. |