ASSOCIATIONS OF SINGLE NUCLEOTIDE POLYMORPHISMS (RS7539542, RS7514221, RS3737884 AND RS1342387) OF RECEPTORS OF ADIPONECTIN WITH HYPERLIPIDEMIA IN PATIENTS WITH TYPE 2 DIABETESAHMED HASSAN KHUDAIR, MAHDI MOHAMMED RIDHA AL-SAHLAWI AND MAJID KADHUM HUSSAIN
Type 2 diabetes is disorder that is characterized by uncontrolled elevation the level of glucose that induce complications that may threat the life and the quality of life in those that suffer from the abnormal action of insult and so may induce many healthy problems that could lead eventually to death this disease induced by many genetic and environmental factors. Some of these genes found in different chromosomes weather coding, non-coding and even un translated region. One of these gene is diponectin receptor one gene. Genetic variant of this receptor may induce a healthy problems and may lead to any diseases like cardiovascular disease, cancer, diabetes and other. To predict whether or not that diponectin receptor1 genetic variants have an effect on biochemical parameters in patient with diabetes. In case control study that conducted in Kufa University, Department of clinical chemistry. eight hundred individuals (400 control volunteer in addition to another 400 DM patients were enrolled in the study. 5 milliliter of blood was taken and divided in to two milliliter that kept in serum tube for biochemical measurements in addition to three milliliter put in EDTA tube for DNA extraction. DNA extraction was done to measure gene variants (SNP) of adiponectin receptor 1 (rs7539542,rs7514221, rs1342387 and rs3737884) followed by amplification by PCRRFLP then electrophoresis by 2 % agarose gel phenotypic measurements was done for SLP, BGL, IR data was analyzed by SPSS version 20. Data indicate that the four SNPs have insignificant effect on BMI but shows different correlations with SLP, insulin resistance and blood glucose level.