GENOTYPIC RESISTANCE PROFILE OF ESCHERICHIA COLI PRODUCING EXTENDED-SPECTRUM BÊTA-LACTAMASE (ESBL) ISOLATED FROM PIGLETS IN ABIDJAN (CÔTE D’IVOIRE)AMINE NATY TADIOGO KONÉ, KOUADIO-NGBESSO NADÈGE, KOUA ATOBLA, INNOCENT KOUAMÉ KOUADIO, FERNIQUE KOUADIO KONAN, BAGUY MOHAMED OUATTARA, MARCELLIN KOFFI DJE, MIREILLE DOSSO, NATHALIE KOUADIO GUESSENND AND ADJÉHI DADIE
ESBL-producing bacteria are a major concern in animal and human health. In livestock, these bacteria have emerged because of the overuse of antibiotics. This study aims to evaluatethe genotypic resistance profile of Escherichia coli ESBL-producing isolated from weaned piglets stools in Abidjan. Escherichia coli were isolated on Mac Conkey medium from 40 stools samples obtained from a farm in AdiopodoumÃ© locality. Then, antibiotic susceptibility test was performed using disk diffusion methodon MÃ¼ller-Hinton agar to screen ESBL-producing Escherichia coli. In order to spot ï¢-lactamase genes (blaTEM, blaSHV, and blaCTX-M), polymerase chain reaction was conducted. Antibiotic susceptibility test reveals that 58 of 330 strains isolatedare ESBL-producing (17.5%). All of these strains were resistant to tetracycline, amoxicillin and piperacillin and all were sensitive to imipenem and colistin. Escherichia coli producing-ESBL also showed relatively high levels of resistance to nalidixic acid (58.7%), sulfamethoxazole-trimethoprim (56.9%) and ciprofloxacin (41.4%). The findings obtained from simplex PCR assay showed that out of collected strains of ESBL-producing Escherichia coli, had 70.7% blaTEM, 20.7% blaCTX-M and 48.3% blaSHV genes. TEM prevalence was high among other types of ESBLs. Escherichia coli ESBL-producing of swine origin could be released into environment and cause public health concerns. It is therefore necessary to set up surveillance plan of ESBL in pig farm.