Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 22, Issue 2, 2020; Page No.(233-238)

MOLECULAR CHARACTERIZATION OF STAPHYLOCOCCUS SP. ISOLATED FROM DIFFERENT TYPES OF CHEESE

WALEED K. ALKHAFAJE, SAFAA M. ALI AND ZAKIA A. OLAMA

Abstract

Cheese production is one of the great Eastmain industries. Among all types of cheese, kariesh, industrial white, old and roomy cheese were investigated as a source of isolating Staphylococcus sp. Antibiotic susceptibility test of 7 antibiotics was tested for the Staphylococcus as evaluated. The selected bacterial isolates were proved to be resistant to Penicilin (P), Chloramphenicol(C), Ofloxacin (OFX), Cefepime (CPM), Meropeneme (MEM), Metronidazole (MTZ), Doxycycline (DO), Ceftazidime (CAZ), Clindamycin (DA), Rifampine (RA), Azetreonum (ATM), Tetracycline (TE) and Trimethoprim-sulfamethoxaz (STX). Molecular identification were approved to confirm the suspected Staphylococcus sp. Molecular studies of total DNA showed that all the selected bacterial isolates have plasmids with size less than 5000bp. Molecular characterization were evaluated using specific PCR (12 pair of primer). Detection of multidrug resistance gene among the selected Staphylococcus sp. isolates were evaluated. Data revealed that TEM gene present in all Staphylococcus isolates, on the other hand (CIT and VIM genes) present in bacteria isolated from white cheese only. Staphylococci isolated from white cheese have KPC, SHV and OXA55 genes. Mececylline gene (Mec) and IPM gene were absent in all tested Staphylococcus isolate, although Staphylococcus isolated from (industrial white cheese, old cheese and roomy cheese) have vancomycin gene (VAN).

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