Ecology, Environment and Conservation Paper

EXTRACTION, ISOLATION AND EVALUATION OF THE ANTI-INFLAMMATORY ACTIVITY OF THE BIOACTIVE COMPONENT PRESENT IN THE BARK OF DELONIX REGIA

Lisha Kurup and Anjali Tiwari

Abstract

The present study deals with the study of anti-inflammatory activity of the extract obtained from the bark of Delonix regia. Soxhlet extraction of dried powder of bark of the tree was done with petroleum ether and methanol. Phytochemical test was carried out and was found that the methanol extract contained alkaloid and flavanoids. The crude extract obtained was then subjected to column chromatography and different fractions eluted were then monitored by TLC. The IR spectral analysis revealed the presence of amide and alkyl halide group in the isolated fraction. In-vitro Anti-inflammatory test was carried out by testing protein denaturation while in-vivo Anti-inflammatory activity was studied by Carrageenan induced paw-edema model. The in-vitro study showed that the crude extract was having more potential against inflammation than the isolated one while in-vivo study concludes that 400 mg/kg gave more significant result than 200 mg/kg.