Ecology, Environment and Conservation Paper

PAHS-DEGRADING BACTERIA ISOLATED FROM OIL CONTAMINATED SOIL OF WESTERN KAZAKHSTAN

E.R. Faizulina, S.A. Aitkeldiyeva, L.G. Tatarkina, M.B. Alimzhanova, Î.N. Auezova, S.T. Daugaliyeva, A.V. Alimbetova, G.A. Spankulova and A.K. Sadanov

Abstract

This work has been aimed at isolating and studying active destructor strains of polycyclic aromatic hydrocarbons. The strains capable of growing on naphthalene and fluorene as the only source of carbon and energy were isolated from oil-contaminated soil of Western Kazakhstan using the Enrichment Culture Technique. Studying the 16S rRNA gene sequence has shown that all active strains belong to the Pseudomonas and Bacillus genera. Using the gas chromatographic method, it has been shown that naphthalene was completely degraded in two to five days, and fluorene – in 15 days. The Bacillus haynesii 21WDT strain has been capable of degrading both naphthalene and fluorene.