LEVELS OF PARASITEMIA AND TNF-α EXPRESSION IN MICE (MUS MUSCULUS) LIVER CELLS INFECTED WITH PLASMODIUM BERGHEI AFTER ADMINISTRATION WITH THE FLESH FRUIT EXTRACT OF PHALERIA MARCOCARPAAnatje Joningsi Pattipeilohy, Pieter Kakisina, Hermalina Sinay, Moch Affandi and Trisnadi Widyaleksono Catur Putranto
Malaria is a disease caused by the Plasmodium parasite and is transmitted to humans through the bite of female Anopheles mosquitoes. The purpose of this study was to determine the effect of the extract of the flesh of the Godâs crown fruit (Phaleriamar cocarpa) with different doses on the level of parasitemia and TNF- Î± expression in mice (Mus musculus) liver cells infected with Plasmodium berghei. The object of research is 25 mice aged 6-8 weeks, with body weight between 20-30 g. The five treatment groups were the negative control group (K-), i.e. mice that were infected with P. berghei without the extract of the flesh of the Godâs crown and the positive control group (K +), i.e. mice that were infected with P. berghei and given anti-malaria suldox drugs. The treatment group (P1) were mice infected with P.berghei and were given extracts of the flesh of the Godâs crown dose of 100 mg/kg BW. The treatment group (P2) were mice infected with P.berghei and were given extracts of the flesh of the Godâs crown dose of 150 mg/kg BW. The treatment group (P3) were mice infected with P. berghei and were given an extract of the flesh of the Godâs crowndose of 200 mg/ kg. The results of the study aimed at observing the expression of TNF-Î± by immonohisto chemical methods in mice liver cells marked with a brown color on hepatocytes. At a dose of 200 mg/kg BW, TNF-Î± expression was not seen on hepatocytes along with an increase in the dose of the extract of the flesh of the Godâs crown fruit (P. macrocarpha).