Ecology, Environment and Conservation Paper

THE EFFECT OF WUNGU LEAF EXTRACT (GRAPTOPHYL LUMPICTUM (L.) GRIFF) AGAINST VAGINAL WALL THICKNESS AND COLLAGEN DENSITY IN THE OVARIECTOMIZED MICE (MUS MUSCULUS)

Listijani Suhargo, Siti Richa Isnaini and Dwi Winarni

Abstract

Menopause is part of a woman’s reproductive physiological condition which causes a decrease in the estrogen hormone synthesis and caused decrease in cell proliferation, and collagen synthesis in the vagina. The purpose of this study was to determine the effect of wungu leaf extract with its estrogenic substance on the thickness and the collagen density of mice vaginal wall that has been ovariectomized. In this study, 24 female mice were divided into 6 groups: normal control group (without ovariectomy and given CMC solution), positive control ovariectomy (esthero solution 0.0104 mg/kg BW), negative control ovariectomy (CMC solution 1g/L), and wungu leaves {Graptophyl lumpictum (L.) Griff} extract treatment groups (10, 20, and 30 mg/kg BW). The mice were given orally once a day for 40 days. All mice were sacrificed, vaginal organs were taken, then histological preparations were made with paraffin method using van Gieson and Weigert’s Iron Hematoxylin staining. Finally, treatment, the parameter analysis was performed statistically at α = 0.05. The results showed that the given of wungu leaves extract could increase the wall thickness and the collagen density of vagina and the optimal dose was 20 mg/kg BW. The conclusion of this study is that wungu leaves extract could prevent vaginal wall damage in pasca ovariectomy or menopause condition.