Ecology, Environment and Conservation Paper

Vol 26, June Suppl. Issue, 2020; Page No.(145-151)

IN VITRO STUDY: THE ADDITION OF ELICITOR CARBOHYDRATES AGAINST THE ACCUMULATION OF ANTHOCYANIN ON A CALLUS DRAGON FRUIT

Indarwati, Sri Arijanti, Jajuk Herawati, Ristani W. Inti and Primawan P. Nugrahadi

Abstract

Plants are the main source of chemical compounds that are used particularly for the pharmaceutical industry, food additives, perfumes. The production of secondary metabolite and elicitation can be used as one of the important tools in order to improve the synthesis of these compounds by in vitro technique. Dragon fruit contains Anthocyanins which are compounds that function as antioxidants; liberate of free radicals and slow the process of ageing/anti-ageing. In dragon fruit plant cell cultures, elicitors have increased metabolic production seconder. Although elicitation has been carried out in a large number of medicinal plants, we extensively research was conducted to improve the content of anthocyanins in callus dragon fruit. The study was conducted using a completely randomized factorial design with two factors, Factor I. Carbohydrate sources; G1 = Glucose, G2 = Fructose, G3 = Sucrose. Factor II. Concentration addition of carbohydrate K1 = 5%, = 10% K2, K3 = 15%, = 20% K4, K5 = 25%; Each treatment was repeated 3 times. Observations showed that the quality of callus formed inclined toward compact. The quantity of callus formed from the treatment source of carbohydrates and carbohydrate concentrations increase with the addition of 15% sucrose (G3K3) tend to produce callus quantity more / bigger. Results of analysis of the content of anthocyanins in callus dragon fruit age 56 dap and 84 dap shows as modifying carbohydrates Media MS with addition sucrose and sucrose concentration of 15% (G3K3) produced anthocyanins content tends to be higher (0.11% and 0.14%) compared to other treatments.

Journal Issues
Vol 26, Issue 2, 2020
Vol 26, June Suppl. Issue, 2020
Vol 26, April Suppl. Issue, 2020
Vol 26, Issue 1, 2020
Vol 26, Feb Suppl. Issue, 2020
Vol 25, Issue 4 2019
Vol 25, Issue 3 2019
Vol 25, Nov Suppl. Issue, 2019
Vol 25, Sept Suppl. Issue, 2019
Vol 25, Aug Suppl. Issue, 2019
Vol 25, July Suppl. Issue, 2019
Vol 25, Issue 2 2019
Vol 25, May Suppl. Issue, 2019
Vol 25, April Suppl. Issue, 2019
Vol 25, Issue 1 2019
Vol 24, Issue 4 2018
Vol 24, Issue 3 2018
Vol 24, Issue 2 2018
Vol 24, Issue 1 2018
Vol 24, March Suppl. Issue 2018
Vol 24, Feb. Suppl. Issue 2018
Vol 23, Issue 4, 2017
Vol 23, Nov. Suppl. Issue 2017
Vol 23, Sept. Suppl. Issue 2017
Vol 23, Issue 2, 2017
Vol 23, Issue 3, 2017
Vol 23, Issue 1, 2017
Vol 23, Feb 2017 Suppl. Issue
Vol 22, Dec 2016 Suppl. Issue
Vol 22, Issue 4, 2016
Vol 22, Sept. Suppl. Issue , 2016
Vol 22, Issue 3, 2016
Vol. 22, June Suppl. Issue 2016
Vol 22, Issue 2, 2016
Vol. 22, April Suppl. Issue 2016
Vol 22, Issue 1, 2016
Vol 21, Issue 4, 2015
Vol. 21 Dec. 2015 Suppl. Issue
Vol. 21 Nov. 2015 Suppl. Issue
Vol 21, Issue 3, 2015
Vol 21, Issue 2, 2015
Vol. 21 Suppl.Issue August 2015
Vol 21.Suppl.Issue June 2015
Vol 21, Issue 1, 2015
Supplement Issue, Dec. 2014
Special Issue-2014
Vol 20, Issue 4, 2014
Vol 20, Issue 3, 2014
Vol 20, Issue 2, 2014
Vol. 20 Issue 01, 2014
Vol. 19 Issue 04, 2013
Vol. 19 Issue 03, 2013
Vol. 19, Issue 02, 2013
Vol. 19, Issue 01, 2013
Vol.18, Issue 04, 2012
Vol.18, Issue 3, 2012
Vol.18, Issue 2, 2012
Vol.18, Issue 1, 2012
Vol.17, Issue 4, 2011
Vol.17, Issue 3, 2011
Vol.17, Issue 2, 2011
Vol.17, Issue 1, 2011
Vol.16, Issue 4, 2010
Vol.16, Issue 3, 2010
Vol.16, Issue 2, 2010
Vol.16, Issue 1, 2010
Vol.15, Issue 04, 2009
Vol.15, Issue 03, 2009
Vol.15, Issue 02, 2009
Vol.15, Issue 1, 2009
Vol.14, Issue 04, 2008
Vol.14, Issue 2-3, 2008
Vol.14, Issue 2-3, 2008
Vol.14, Issue 1, 2008
Vol.14, Issue 2-3, 2008
Vol.13, Issue 04, 2007
Vol.13, Issue 2, 2007
Vol.13, Issue 1, 2007
Vol.12, Issue 4, 2006
Vol.12, Issue 3, 2006
Vol.12, Issue 2, 2006
Vol.12, Issue 1, 2006
Vol.12, Issue 01, 2006
Vol.11, Issue 3,4, 2005
Vol.11, Issue 2, 2005
Vol.11, Issue 1, 2005
Vol.10, Issue 04, 2004
Vol.10, Issue 03, 2004
Vol.10, Issue 02, 2004
Vol.10, Issue 01, 2004
Vol.09, Issue 04, 2003
Vol.09, Issue 03, 2003
Vol.09, Issue 02, 2003
Vol.08, Issue 04, 2002
Vol.08, Issue 03, 2002
Vol.08, Issue 01, 2002
Vol.07, Issue 04, 2001
Vol.07, Issue 03, 2001
Vol.07, Issue 02, 2001
Vol.07, Issue 01, 2001
Vol.06, Issue 04, 2000
Vol.06, Issue 03, 2000
Vol.06, Issue 02, 2000
Vol.06, Issue 01, 2000
Vol.05, Issue 04, 1999
Vol.05, Issue 03, 1999
Vol.05, Issue 02, 1999
Vol.05, Issue 01, 1999
Vol.04, Issue 1,2, 1998
Vol.03, Issue 3,4, 1997
Vol.03, Issue 01, 1997
Vol.02, Issue 1,2, 1996
Vol.01, Issue 14, 1995
Looking for Past Issues? Click here to get them!!