BLOOD CELLS AS BIOMARKERS OF KOI (CYPRINUS CARPIO) INFECTED BY MYXOBOLUS SP. WITH TREATMENT OF DIFLUBENZURON IN THE WATER CULTURE OF QUALITYUun Yanuhar, Kurnia Susilowati, Nur Sakinah Junirahma, Nico Rahman Caesar and Muhammad Musa
Myxobolus sp. often attacks koi (Cyprinus carpio). Therefore, to cure that disease using natural or chemicals treatment, such as Diflubenzuron. Haematology is one part of the fish that can be used as an indicator to determine the health level of fish. The research to determine the status of haematology after being treated by Diflubenzuron. The method used was experimental with 5 treatments and Diflubenzuron dose that used is A (0.01 mg/5 L of water), B (0.02 mg/5 L of water), and C (0.03 mg/5 L of water). Haematological parameters were erythrocytes, leukocytes, haemoglobin, and hematocrit. The data analysis was done using ANOVA. The results of the research on the Myxobolus sp. with Diflubenzuron 0.02 g treatment is the best treatment. The erythrocytes at doses A lower (2.29 x 106- 2.40 x 106 cell/mm3 ) than dose B and C because higher erythrocyte proves to indicate stress. Erythrocytes obtained is directly proportional to the hematocrit value. Dose A of leukocytes also lower (125.800-131.250 cells/mm3 ) than dose B and C because the higher leukocytes prove that fish will try to protect the body from foreign objects. The results of water quality were obtained, the temperature 24.9 o C; pH 7.79; dissolved oxygen 5.8 mg/L; CO2 4.9 mg/L. Based on the results obtained values of temperature, pH, DO, and CO2 still in optimal condition because the water quality is still controlled with sufficient aeration. In conclusion, the Diflubenzuron that is given to Koi fish infected Myxobolus sp. is a very significant effect on haematological parameters.