Ecology, Environment and Conservation Paper

Vol 26, June Suppl. Issue, 2020; Page No.(9-13)

THE EFFECT OF IBA AND ETHEPON ON GROWTH AND SAPONIN CONTENT OF TALINUM PANICULATUM GAERTN. ADVENTITIOUS ROOT IN VITRO

Nindi Novia Erin, Junairiah and Yosephine Sri Wulan Manuhara

Abstract

Root of Talinum paniculatum is a raw material that is used as medicinal raw materials because it contains active compound of saponin which can increases the body’s resistance to disease. Growth of T. paniculatum root in natural habitat was slow, so we want to improve production of T. paniculatum root through in vitro culture. This research aims to determine the effect of IBA and ethephon to growth and saponin content of adventitious root T. paniculatum. Leaf explants were planted in solid MS medium with combination of IBA 0; 1; 2; 3 mg/L and ethephon 0; 0,1; 1 mg/L. Culture was incubated for 6 weeks. Data’s root growth such as time of root formation, number of roots, length of root, fresh weight of root, and dry weight of root were analyzed using non parametric test (significance level 0.05). Data’s saponin content such as stain area and thickness stain were analyzed semi quantitatively using Thin Layer Chromatography (TLC). The results showed that the treatment of IBA 1 mg/L + ethephon 0.1 mg/L produced adventitious roots with the fastest average time of day 7. The treatment of IBA 2 mg/L + ethephon 1 mg/L produced the highest average number of roots was 7.33 roots, the longest average length of root was 1.59 cm, also the highest average fresh weight and dry weight of root were 29.80 mg and 2.70 mg. The highest saponin content based on stain area on TLC plate respectively resulted by the treatment of IBA 1 mg/L + ethephon 1 mg/L was 0.38 cm2/0.01 g dry weight and IBA 2 mg/L + ethephon 1 mg/L for thickness stain.

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