Ecology, Environment and Conservation Paper

Vol 26, April Suppl. Issue, 2020; Page No.(205-208)

LARVICIDAL ACTIVITY OF METHANOL FRACTIONS FROM CARICAPAPAYA LEAVES EXTRACT AGAINST AEDES AEGYPTI

Tri Puji Lestari Sudarwati, M.A.H. Ferry Fernanda, Hilya Nur Imtihani and Intan A. Pratiwi

Abstract

Dengue Haemorrhagic Fever (DHF) is a disease caused by dengue virus which is transmitted through Aedes aegypti mosquito bite. Efforts to control the widespread of the vectors have been made using biological agents and also chemical compounds. Chemicals known as a standard protocol have raised concerns about resistance and dengirus to the environment. Hence, the present study was aimed to explore the larvicidal activity of papaya (Carica papaya) leaf extract against Aedes aegypti larvae in regards to the optimum concentration and time exposure. Preparation the obtained extract was diluted to make a serial concentration.These solutions were made by pipetting 0.65 mL, 1.25 mL, 2.5 mL, 5.0 mL, and 10.0 mL of extract into 10.0mL volumetric flasks and dilute with distilled water. The test solution was poured into a glass jar contained 90 mL of distilled water and filled with 20 third instar larvae. Each experiment was replicated four times.The larval mortality was recorded in 24h and calculated as a percentage of total larvae used in the experiment.The table above shows the value of LC 50 from toxicity assay of papaya leaf extract. According LC50 is 4929,344 ppm. Based on these results, papaya leaves have the ability to Aedes aegypti larva side so that it can help in breaking the chain of development of Aedes aegypti.

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