CORN COB HYDROLYZATE FROM PENICILLIUM CITRINUM H9 AS AN ALTERNATIVE SUBSTRATE FOR BIO SURFACTANT PRODUCTION BY HYDROCARBONOCLASTIC BACTERIAFatimah, Silvia Kurnia Sari, Nastiti Trikurniadewi, Syahriar Nur Maulana Malik Ibrahim,Ana Mariatul Khiftiyah, Khudrotul Nisa Indriyasari, Tri Nurhariyati, Tini Surtiningsih and Hanif Yuliani, Ni’matuzahroh
Corn cob is a potential agricultural waste that can be utilized as raw materials in bio industries. Commonly, corn cob is regarded as agricultural waste and would be eliminated by burning, however this elimination method would produce a toxic fume that polluted the atmosphere. So, new utilization methods of corn cob need to be developed. This research aimed to reveal the potency of corn cob hydrolyzate (CCH) that produced enzymatically using Penicillium citrinum H9 as substrate for bio surfactant production. The CCH was used as growth substrate for bio surfactant production by seven indigenous bacteria of Balongan oil sludge. All isolates were tested the growth and the capability of bio surfactant production through surface tension and emulsification activity measurement. One potential isolate was obtained from this observation. BP1(5) isolate has a lowering surface tension at 56.64Â±4.63 mN/m in five days incubation and the growth rate was 0.52âcell/hour. The best bio surfactant product was obtained at concentration 200 ppm of CCH in five days incubation by 23.93Â±3.56 mN/m in surface tension reductions but the emulsification activity was not detected.This research showed that the CCH be utilized as substrate for bio surfactant production by hydrocarbonoclastic bacteria.