Ecology, Environment and Conservation Paper

ISOLATION AND CHARACTERIZATION OF COCKROACH ENDOSYMBIONT BACTERIA WITH POTENTIAL TO PRODUCE HYDROLYTIC ENZYME OF ORGANIC MATERIAL

Ni’matuzahroh, Nastiti Trikurniadewi, Syahriar Nur Maulana Malik Ibrahim,Achmad Zainal Abidin, Ana Mariatul Khiftiyah, Silvia Kurnia Sari,Eka Narendra Nuswantara, Fawwas Nurmansyah, Muhammad Aufar Rizki Wildan Rahman,Habibah Lailatul Maghfirah, Miftahul

Abstract

Cockroaches are one of the most popular decomposers in organic matter processing. Its ability to decompose organic matter is supported by the association with the digestive tract microbiota. Microbial exploration of the cockroach gastrointestinal tract is still rarely studied, especially in Indonesia. This study aims to obtain potential bacterial isolates that produce enzymes that can decompose of organic matter derived from cockroach midgut. Cockroach samples were obtained from household organic wastes composts. Midguts of cockroach sample were surgically removed, mashed aseptically, and inoculated into nutrient agar medium. Endosymbiont bacteria grown in the media then purified, characterized, and tested for enzymatic abilities such as amylase, protease, lipase and cellulase. The abundance of cockroach midgut endosymbiont bacteria was detected as much as 1.1 x 104 CFU/mL. A total of 20 endosymbiont bacterial isolates had different characters and had the ability to hydrolyze starch, cellulose, protein, and lipid. Three potential bacterial isolates in hydrolyzing starch, cellulose, protein, and lipid were EKA 4, EKA 8, and EKA 20. Cockroach endosymbiont isolates are potential to be applied for organic waste treatment.