DIVERSITY OF MODERATELY THERMO-ALKALIPHILE BACTERIA PRODUCING AMYLASE ORIGINATED FROM PARIANGAN HOT SPRING, WEST SUMATRA, INDONESIAGustina Indriati, Ruth Rize Pass Megahati and Dwi Hilda Putri
Moderately thermo-alkaliphile bacteria are bacteria originating from hot springs that have a pH above 8. Moderately thermo-alkaliphile bacteria are currently used as a source of various enzymes, such as lipases,proteases, and amylase. Amylase is an enzyme that can hydrolyze polysaccharides into simple sugar.Amylase produced by moderately thermo-alkaliphile bacteria plays a role in various industrial fields, such as in the leather tanning industry, biscuits production, textiles, and as detergent additives. In the detergent industry,amylase is needed which is resistant to high temperatures and pH. The worldâs need for amylase continues to increase to 65% of the needs of enzymes worldwide. Isolation of moderately thermo-alkaliphile bacteria from Pariangan hot springs of West Sumatra has been successfully carried out and 17 bacterial isolates were obtained. From 17 isolates, there were 3 isolates of bacteria that produced amylase, namely PR 1.2, PR2.3, and PR 3.1. The characterization and analysis of the biochemical properties of the three isolates are not known. The aims of the study were to the identification of moderately thermo-alkaliphile bacteria producing amylase originated from Pariangan hot spring, West Sumatra, Indonesia. The method used is characterization of isolates (color, shape, edges, and elevation), Gram staining (morphology and spores), biochemical properties of isolates (TSIA, Catalase, Oxidase, Indole, Urea Citrate, Lactose, Glucose, Sucrose, Mannitol,MR, VP, OF, Arabinose, Nitrate, and Gelatin. The results of this study showed that isolates of PR 1.2 bacteria,PR 2.3 belonging to Bacillus sp and isolates of PR 3.1 bacteria belonging to Enterobacter sp.