Ecology, Environment and Conservation Paper

Vol 26, April Suppl. Issue, 2020; Page No.(77-80)

DIVERSITY OF MODERATELY THERMO-ALKALIPHILE BACTERIA PRODUCING AMYLASE ORIGINATED FROM PARIANGAN HOT SPRING, WEST SUMATRA, INDONESIA

Gustina Indriati, Ruth Rize Pass Megahati and Dwi Hilda Putri

Abstract

Moderately thermo-alkaliphile bacteria are bacteria originating from hot springs that have a pH above 8. Moderately thermo-alkaliphile bacteria are currently used as a source of various enzymes, such as lipases,proteases, and amylase. Amylase is an enzyme that can hydrolyze polysaccharides into simple sugar.Amylase produced by moderately thermo-alkaliphile bacteria plays a role in various industrial fields, such as in the leather tanning industry, biscuits production, textiles, and as detergent additives. In the detergent industry,amylase is needed which is resistant to high temperatures and pH. The world’s need for amylase continues to increase to 65% of the needs of enzymes worldwide. Isolation of moderately thermo-alkaliphile bacteria from Pariangan hot springs of West Sumatra has been successfully carried out and 17 bacterial isolates were obtained. From 17 isolates, there were 3 isolates of bacteria that produced amylase, namely PR 1.2, PR2.3, and PR 3.1. The characterization and analysis of the biochemical properties of the three isolates are not known. The aims of the study were to the identification of moderately thermo-alkaliphile bacteria producing amylase originated from Pariangan hot spring, West Sumatra, Indonesia. The method used is characterization of isolates (color, shape, edges, and elevation), Gram staining (morphology and spores), biochemical properties of isolates (TSIA, Catalase, Oxidase, Indole, Urea Citrate, Lactose, Glucose, Sucrose, Mannitol,MR, VP, OF, Arabinose, Nitrate, and Gelatin. The results of this study showed that isolates of PR 1.2 bacteria,PR 2.3 belonging to Bacillus sp and isolates of PR 3.1 bacteria belonging to Enterobacter sp.

Journal Issues
Vol 26, Issue 2, 2020
Vol 26, June Suppl. Issue, 2020
Vol 26, April Suppl. Issue, 2020
Vol 26, Issue 1, 2020
Vol 26, Feb Suppl. Issue, 2020
Vol 25, Issue 4 2019
Vol 25, Issue 3 2019
Vol 25, Nov Suppl. Issue, 2019
Vol 25, Sept Suppl. Issue, 2019
Vol 25, Aug Suppl. Issue, 2019
Vol 25, July Suppl. Issue, 2019
Vol 25, Issue 2 2019
Vol 25, May Suppl. Issue, 2019
Vol 25, April Suppl. Issue, 2019
Vol 25, Issue 1 2019
Vol 24, Issue 4 2018
Vol 24, Issue 3 2018
Vol 24, Issue 2 2018
Vol 24, Issue 1 2018
Vol 24, March Suppl. Issue 2018
Vol 24, Feb. Suppl. Issue 2018
Vol 23, Issue 4, 2017
Vol 23, Nov. Suppl. Issue 2017
Vol 23, Sept. Suppl. Issue 2017
Vol 23, Issue 2, 2017
Vol 23, Issue 3, 2017
Vol 23, Issue 1, 2017
Vol 23, Feb 2017 Suppl. Issue
Vol 22, Dec 2016 Suppl. Issue
Vol 22, Issue 4, 2016
Vol 22, Sept. Suppl. Issue , 2016
Vol 22, Issue 3, 2016
Vol. 22, June Suppl. Issue 2016
Vol 22, Issue 2, 2016
Vol. 22, April Suppl. Issue 2016
Vol 22, Issue 1, 2016
Vol 21, Issue 4, 2015
Vol. 21 Dec. 2015 Suppl. Issue
Vol. 21 Nov. 2015 Suppl. Issue
Vol 21, Issue 3, 2015
Vol 21, Issue 2, 2015
Vol. 21 Suppl.Issue August 2015
Vol 21.Suppl.Issue June 2015
Vol 21, Issue 1, 2015
Supplement Issue, Dec. 2014
Special Issue-2014
Vol 20, Issue 4, 2014
Vol 20, Issue 3, 2014
Vol 20, Issue 2, 2014
Vol. 20 Issue 01, 2014
Vol. 19 Issue 04, 2013
Vol. 19 Issue 03, 2013
Vol. 19, Issue 02, 2013
Vol. 19, Issue 01, 2013
Vol.18, Issue 04, 2012
Vol.18, Issue 3, 2012
Vol.18, Issue 2, 2012
Vol.18, Issue 1, 2012
Vol.17, Issue 4, 2011
Vol.17, Issue 3, 2011
Vol.17, Issue 2, 2011
Vol.17, Issue 1, 2011
Vol.16, Issue 4, 2010
Vol.16, Issue 3, 2010
Vol.16, Issue 2, 2010
Vol.16, Issue 1, 2010
Vol.15, Issue 04, 2009
Vol.15, Issue 03, 2009
Vol.15, Issue 02, 2009
Vol.15, Issue 1, 2009
Vol.14, Issue 04, 2008
Vol.14, Issue 2-3, 2008
Vol.14, Issue 2-3, 2008
Vol.14, Issue 1, 2008
Vol.14, Issue 2-3, 2008
Vol.13, Issue 04, 2007
Vol.13, Issue 2, 2007
Vol.13, Issue 1, 2007
Vol.12, Issue 4, 2006
Vol.12, Issue 3, 2006
Vol.12, Issue 2, 2006
Vol.12, Issue 1, 2006
Vol.12, Issue 01, 2006
Vol.11, Issue 3,4, 2005
Vol.11, Issue 2, 2005
Vol.11, Issue 1, 2005
Vol.10, Issue 04, 2004
Vol.10, Issue 03, 2004
Vol.10, Issue 02, 2004
Vol.10, Issue 01, 2004
Vol.09, Issue 04, 2003
Vol.09, Issue 03, 2003
Vol.09, Issue 02, 2003
Vol.08, Issue 04, 2002
Vol.08, Issue 03, 2002
Vol.08, Issue 01, 2002
Vol.07, Issue 04, 2001
Vol.07, Issue 03, 2001
Vol.07, Issue 02, 2001
Vol.07, Issue 01, 2001
Vol.06, Issue 04, 2000
Vol.06, Issue 03, 2000
Vol.06, Issue 02, 2000
Vol.06, Issue 01, 2000
Vol.05, Issue 04, 1999
Vol.05, Issue 03, 1999
Vol.05, Issue 02, 1999
Vol.05, Issue 01, 1999
Vol.04, Issue 1,2, 1998
Vol.03, Issue 3,4, 1997
Vol.03, Issue 01, 1997
Vol.02, Issue 1,2, 1996
Vol.01, Issue 14, 1995
Looking for Past Issues? Click here to get them!!