THE EFFECT OF OKRA PODS (ABELMOSCHUS ESCULENTUS L.) METHANOL EXTRACT ON WHITE BLOOD CELL COUNT, PHAGOCYTIC ACTIVITY, AND IFN-GAMMA LEVEL IN MUS MUSCULUS EXPOSED SODIUM NITRITESri Puji Astuti Wahyuningsih, Siti Richa Isnaini, Dwi Winarni, Win Darmanto, Manikya Pramudya,Anni Mar’atun Nafi’ah, Khoirunnisa, Elma Sakinatus Sajidah and Baiq Naili Dewi AtikaAbstract Okra is widely used as a vegetable and known have health benefits because it contains high antioxidants.The purpose of this study was to determine antioxidant potential of okra pods methanol extract (OPM) on immune response (white blood cell count, activity phagocytic, and IFN-gamma level) of Mus musculus exposed sodium nitrite (NaNO 2). Twenty four male mice (BALB/c strain, 8-10 weeks, 25-30 g body weight were divided into 6 groups: normal control group (exposed distilled water), negative control (exposed NaNO 250 mg/kg body weight), and OPM treatment (exposed to NaNO 2 and OPM 50, 100, 200 and 400 mg/kg BW simultaneously). The mice was administrated orally both NaNO2 and OPM once a day for 19 days. Finally treatment, the parameter analysis was performed statistically at P = 0.05. The results showed that the administration of OPM could increase white blood cell count (WBCs), phagocytic activity, and IFN-gamma level significantly. The optimal dose of OPM is 50 mg/kg BW. The conclusion of this study is that the administration of OPM can increase the immune response (white blood cell count, activity phagocytic, and IFN-gamma levels) of Mus musculus exposed NaNO2. |