Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 22, Issue 1, 2020; Page No.(107–110)

ANALYSIS OF DIMETHOATE DEGRADATION BY KOCURIA TURFANENSIS USING GC-MS

J. BAROT AND K. CHAUDHARI

Abstract

Pesticide is an essential factor in agricultural fields of India. Because of its beneficial properties dimethoate is most commonly used organophosphorus pesticide. At the same time unwise use of dimethoate exerts hazardous effects on ecosystem via bioaccumulation and biomagnifications. However, effective removal or degrading technique has to be identified. Hence, the present study was aimed to carry out dimethoate degradation by potential microorganism. A novel dimethoate degrading bacterial strain was isolated from agriculture soil. The strain utilizes dimethoate as sole carbon source. On the basis of morphological, biochemical characteristic and 16S rRNA gene sequence analysis the strain was identified as Kocuria turfanensis. Dimethoate contaminated soil was treated with Kocuria turfanensis for degradation and degradation products were identified using gas chromatography – mass spectrometry (GC-MS) analysis. The result indicated that the Kocuria strain degraded 78.22% dimethoate.

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