Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 22, Issue 1, 2020; Page No.(57-62)

A PHYLOGENETIC STUDY OF OIL DEGRADING BACTERIA ISOLATED FROM SELECTED STATIONS IN BASRAH CITY

FADHIL N. AL-KANANY AND RASHA M. OTHMAN

Abstract

The target of this work was to isolate and identify indigenous bacteria from local oil-contaminated sites associated with petroleum industries, then study the relationship among the oil degrading bacteria as well as to the identification of the dominant bacterial community in these areas. In the current study the enrichment culture technique was employed using basal mineral salts medium (MSM) broth supported with crude oil as a sole source of carbon to isolate the desired microorganisms. Depending on the Biochemical and Molecular criteria as well as the genes encoding 16S rRNA, 17 isolates have been identified and characterized according to the genotypic approaches. The 16S rRNA encoding gene was used because its conservative during generations. The successfully identified genera were Acinetobacter radioresistens, Pseudomonas oryzihabitans, Aeromonashydrophila, Pseudomonas putida, Micrococcus luteus, Pseudomonas aeruginosa, Pseudomonas guariconensis, Achromobacter xylosoxidans, Enterobacter sp., Klebsiella sp., Staphylococcus sp., Bacillus foraminis, Exiguobacterium sp., Bacillus firmus, Brevibacillus brevis, Brevibacillus brevis, Stenotrophomonas sp., Pseudomonas stutzeri. The percentage of similarity was very closely related [over 99% of all reported valid genera except Bacillus foraminis (96.77) and Pseudomonas stutzeri (93.88%)] and all analysis sequences were having Expected (E) value 0.0, which means the matches were significant.

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