Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 22, Issue 1, 2020; Page No.(7-14)

ANTIBACTERIAL ACTIVITY AND GC-MS ANALYSIS OF TRICHAPTUM BIFORME EXTRACT

YAKHLEF DJAMILA, DIB SOULEF AND FORTAS ZOHRA

Abstract

In our study, mushrooms called Trichaptum biforme are harvested in west forest of Algeria in order to research their antibacterial activity. Description of mushrooms is carried out by morphological and microscopic methods. Extract is obtained by Soxhlet from the dried sporophores of Trichaptum biforme. This extract is tested in vitro, in Petri dishes, on the growth of a Gram + and Gram- bacteria: Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The results obtained showed a remarkable inhibitory effect of the Trichaptum biforme extract on the growth of the two pathogenic bacterial strains tested. The antibacterial activity of the extract towards the bacterial strains tested by the disc diffusion method has shown that this extract is biologically bacteriostatic. Analysis of the extract by GC-MS allowed the identification of 30 components. Octodrine, caryophyllene oxide, phenolic compounds, fatty acids and fatty acids esters were noted as key compounds in the Trichaptum biforme extract, which were perhaps causing the antibacterial activity. Trichaptum biforme are are therefore interesting candidates for obtaining antibacterial bioactives substances with therapeutic interest.

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