ISOLATION AND MOLECULAR IDENTIFICATION OF BACTERIOCIN-PRODUCING LACTIC ACID BACTERIA FROM FISHERIES WASTEP.H. RIYADI, ROMADHON AND T.W. AGUSTINIAbstract The present study aimed to isolate and identify the bacteriosin-producing lactic acid bacteria (LAB) from fisheries waste by using molecular identification. LAB was isolated from fisheries waste (smoked fish, presto milk and frozen fillets) by dilution series-pour-plate techniques. The obtained isolate were then screened by qualitative, semi-quantitative and quantitative analysis. Bacterial characterization was carried out using morphology, biochemistry, vitex, physiology, morphology, pathogenic, antimicrobial and molecular test analysis. Molecular identification of LAB was carried out using the neighbor-joining method by MEGA 5 Software. All analyses were performed on a bootstrapped date 1000 replicates. Total of 40 isolates was obtained from screening test of LAB and these isolates consist of 12 isolates from smoked fish waste, 11 isolates from presto-milkfish waste and 17 isolates from freezing fish waste. The screening of bacteriocin extract was found 2 isolates of smoked fish waste, 2 isolates of presto-milk fish waste and 2 isolates of freezing fish waste. Molecular identification using 16S rRNA sequences showed that LA01 isolates had the closest relationship with Pediococcus accidilactici DSM 20284 with nucleotide similarity of 98.63%. These results suggested that bacteriocin from LAB fishery waste (smoked fish, presto milk and frozen fillets) has the potential activity as a food preservative. |