Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 4, 2019; Page No.(950-959)

BOROJOA PATINOI CELL CULTURE FOR THE PRODUCTION OF COMPOUNDS WITH ANTICANCER ACTIVITY

ANNY D. MARTÍNEZ-MIRA, AÍDA HURTADO-MOSQUERA, FERNANDO OROZCO-SÁNCHEZ AND RODRIGO A. HOYOS S.

Abstract

Borojó (Borojoa patinoi) is an arboreal species native to Colombia, used ancestrally for wound healing, pulmonary problems and hypertension control. In this research an in vitro culture of borojó cells in suspension was established in order to evaluate its potential in the production of metabolites of pharmaceutical interest, specifically in cancer. Thus, different culture media (MS, B5, and White) and different plugs in the shake flask (cotton, foam, and aluminum foil plugs) were evaluated for B. patinoi cell culture growth in vitro. No differences in cell growth were found using the culture media and plugs evaluated. The specific growth rate was 0.11 + 0.01 day-1 with a doubling time of 6.9 + 0.9 days. MTT test showed the cytotoxic effect of crude extracts of B. patinoi cells on Jurkat and MCF7 tumor cell lines. The extracts obtained with hexane and ethyl acetate at 150 μg/mL reduced cancer cells viability between 45 and 57%. Selectivity towards tumor cells was observed, presenting a greater effect in cancer cells. Furthermore, significant clastogenic damage (27.8%) to Jurkat cells DNA. Finally, chlorogenic acid, coumarins, terpenes, saponins, and sesquiterpene lactones, secondary metabolites of pharmaceutical interest were identified in B. patinoi cells. To our knowledge, this is the first report on the establishment of B. patinoi cell cultures in suspension and evaluation of its potential for obtaining compounds, specifically anticancer

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