Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 4, 2019; Page No.(941-944)

DETECTION OF HELICOBACTER PYLORI BY POLYMERASE CHAIN REACTION FROM FECES OF ASYMPTOMATIC PATIENTS

JHOAN GUAMÁN, FAVIAN BAYAS-MOREJÓN, RIVELIÑO RAMÓN-CURAY, JAGGER SEGURA-OCHOA, IVAN GARCÍA-CÁCERES AND DARWIN NUÑEZ

Abstract

The objective of this work was to make a diagnosis of the presence of Helicobacter pylori in human biological samples (feces). To this end, the samples were selected from 50 outpatients of the Hospital Alfredo Noboa Montenegro General (ANMH) from Guaranda and María Auxiliadora private laboratory from San Miguel de Bolívar in Ecuador with apparent symptomatology to the presence of H. pylori. The samples were processed and analyzed by polymerase chain reaction (PCR). After the data collection through the clinical record, the average age was 30 years (range 5 - 65 years). Through molecular analysis, 11 samples: 4 of 16 from men and 7 of 34 from women were positive for H. pylori, showing in the gel the characteristic 394-bp band. In this work, the PCR technique has proved to be an effective methodology for the detection of H. pylori in biological samples.

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