PRELIMINARY STUDY: IDENTIFICATION OF DNA VARIATION WITH SNP NUMBERS RS1137101 AND RS8050136 IN PATIENT’S TYPE 2 DIABETES MELLITUS AT SALSABILA CLINIC BOGOR – INDONESIACHRIS ADHIYANTO, NARILA MUTIA NASIR, FLORI R. SARI, RISFY GUSTI PAMUNGKAS, INTAN AZIS, ZETI HARRIYATI, WITRI ARDINI, HARI HENDARTO, NOUVAL SHAHAB, ENDAH WULANDARI, KARYADI AND FASE BADRIAH Faculty ofAbstract Type 2 Diabetes Mellitus (T2DM) has become a worldwide pandemic. Data from Indonesia Basic Health Research show the prevalence of diabetes in Indonesia in 2017 is 6.3% which ranked 6th in the world. The Genome-Wide Association Studies (GWAS) has obtained more than 250 genetic loci that are assumed to play a role in the pathogenesis of obesity and T2DM. We have identified the DNA variation in T2DM patients, specifically the LEPR and FTO genes that thought to be linked with T2DM. We chose Rs 1137101 (LEPR Q223R, exon 6 A>G) and rs8050136 (FT0 intron variant C>A) because these SNP variations are common in the Asian region. The result show T2DM subjects had a higher genotype GG for LEPR Rs1137101, and AC for FTO Rs8050136, and it had associated between allele risk Rs1137101 (G) and Rs 8050136 (A) (p<0.0001). In conclusion the research found associated between SNP Rs1137101 and Rs 8050136 in patients T2DM. |