Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 4, 2019; Page No.(846-850)

BIOACTIVE CONTENT AND ANTIOXIDANT ACTIVITY OF ALBEDO POMELO (CITRUS GRANDIS, C. MAXIMA) EXTRACT USING A METHOD OF 2,2-DHIPENIL-1-PICRYLHYDRAZYL (DPPH) IN VARIOUS TYPES OF EXTRACTION SOLVENT

S. SURYANINGSIH*, M. DJALI, B. MUSLIM AND R. ADISAPUTRA

Abstract

Pomelo production has reached 110,000 tones annually and almost 50% albedo pomelo has not been fully utilized. Testing the phenol bioactive and flavonoid content of the albedo pomelo as well as their antioxidant activity is important. Determining the phenol total using the method of DPPH (1,1-diphenyl- 2picrylhydrazyl), flavonoid total using quercetine as comparison and antioxidant activity of various types of extraction solvents by spectrophotometry UV vis. Sample was taken randomly using complete random design. Data obtained were analyzed with the analysis of variance using the F test. Young albedo extract has total phenol of higher than that of mature albedo extract, i.e., 2.04>1.94 g/100 g. Total flavonoid content of mature albedo extract is higher than young albedo extract, i.e., 0.18>0.10 g/100 g. Ethyl acetate extract has the strongest antioxidant activity with the least IC value of 7.71 ppm compared to that of other solvents. The albedo pomelo extract contain phenolic bioactive compound as well as flavonoid, ethyl acetate extract being the strongest in antioxidant activity.

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