Ecology, Environment and Conservation Paper

Vol 25, Issue 4 2019; Page No.(1902-1906)

THE EFFECT OF LAND USE ON THE DIVERSITY OF THE SOILS BACTERIAL COMMUNITIES

I.E. Smirnova, A.K. Sadanov and S.T. Daugaliyeva

Abstract

Natural soils have been increasingly transformed into agricultural land, which leads to a reduction of the natural flora, changes in the composition of soil nutrients, and may affect the microbial diversity. The diversity of the bacterial communities in the two sites with the same soil type but with different land use was studied: virgin lands and cultivated soil. The study was performed by the method of high throughput sequencing of 16S rRNA amplicons of the bacterial gene with the use of the Ilumina MiSeq unit (USA). It has been found that land use reduces the diversity of the bacterial community at the level of phyla and lower taxonomic units. The greatest effect of land use was noted on the Proteobacteria and Firmicutes phyla. In a bacterial community of virgin soils, phylum Proteobacteria, class 〈-Proteobacteria was dominating, in cultivated soil - phylum Firmicutes, class Bacilli, which was resistant to negative factors.

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