Ecology, Environment and Conservation Paper

Vol 25, Issue 4 2019; Page No.(1661-1666)

IDENTIFICATION OF PHYTOPATHOGENIC BACTERIA OF GENUS PSEUDOMONAS USING THE REAL-TIME PCR METHOD

Indira Saltanovna Beishova, Alexandr Mikhailovich Kovalchuk, Tatyana Vladimirovna Poddudinskaya, Vadim Aleksandrovich Ulyanov and Kuantar Daulenovich Alikhanov

Abstract

In increasing the yield of grain and legume crops, an important role is played by their protection from diseases that often result in a significant reduction of crop production, deterioration of quality, and sometimes harvest failure. One of the reasons of harvest failure is the presence of pathogenic bacteria species. Such pathogenic bacteria species include Pseudomonas syringae, Pseudomonas pisi Sackett, and Pseudomonas holci Kendrick. Detection and taxonomic identification of the pathogenic bacteria is important, and almost indispensable not only in agriculture but also in other areas, such as food safety and environmental monitoring. In this research, these bacteria were identified using the method based on real-time PCR, with a matching pair of primers and a probe, which allowed identifying bacteria of genus Pseudomonas wide spread on the territory of Kazakhstan: Pseudomonas syringae, Pseudomonas pisi Sackett, and Pseudomonas holci Kendrick. Identification of bacteria is based on PCR amplification of DNA fragments using specific primers that have been developed based on the gene of subunit B of DNA gyrase.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 26, Aug Suppl. Issue, 2020
Vol 26, Issue 2, 2020
Vol 26, June Suppl. Issue, 2020
Vol 26, April Suppl. Issue, 2020
Vol 26, Issue 1, 2020
Vol 26, Feb Suppl. Issue, 2020
Vol 25, Issue 4 2019
Vol 25, Issue 3 2019
Vol 25, Nov Suppl. Issue, 2019
Vol 25, Sept Suppl. Issue, 2019
Vol 25, Aug Suppl. Issue, 2019
Vol 25, July Suppl. Issue, 2019
Vol 25, Issue 2 2019
Vol 25, May Suppl. Issue, 2019
Vol 25, April Suppl. Issue, 2019
Vol 25, Issue 1 2019
Vol 24, Issue 4 2018
Vol 24, Issue 3 2018
Vol 24, Issue 2 2018
Vol 24, Issue 1 2018
Vol 24, March Suppl. Issue 2018
Vol 24, Feb. Suppl. Issue 2018
Vol 23, Issue 4, 2017
Vol 23, Nov. Suppl. Issue 2017
Vol 23, Sept. Suppl. Issue 2017
Vol 23, Issue 2, 2017
Vol 23, Issue 3, 2017
Vol 23, Issue 1, 2017
Vol 23, Feb 2017 Suppl. Issue
Vol 22, Dec 2016 Suppl. Issue
Vol 22, Issue 4, 2016
Vol 22, Sept. Suppl. Issue , 2016
Vol 22, Issue 3, 2016
Vol. 22, June Suppl. Issue 2016
Vol 22, Issue 2, 2016
Vol. 22, April Suppl. Issue 2016
Vol 22, Issue 1, 2016
Vol 21, Issue 4, 2015
Vol. 21 Dec. 2015 Suppl. Issue
Vol. 21 Nov. 2015 Suppl. Issue
Vol 21, Issue 3, 2015
Vol 21, Issue 2, 2015
Vol. 21 Suppl.Issue August 2015
Vol 21.Suppl.Issue June 2015
Vol 21, Issue 1, 2015
Supplement Issue, Dec. 2014
Special Issue-2014
Vol 20, Issue 4, 2014
Vol 20, Issue 3, 2014
Vol 20, Issue 2, 2014
Vol. 20 Issue 01, 2014
Vol. 19 Issue 04, 2013
Vol. 19 Issue 03, 2013
Vol. 19, Issue 02, 2013
Vol. 19, Issue 01, 2013
Vol.18, Issue 04, 2012
Vol.18, Issue 3, 2012
Vol.18, Issue 2, 2012
Vol.18, Issue 1, 2012
Vol.17, Issue 4, 2011
Vol.17, Issue 3, 2011
Vol.17, Issue 2, 2011
Vol.17, Issue 1, 2011
Vol.16, Issue 4, 2010
Vol.16, Issue 3, 2010
Vol.16, Issue 2, 2010
Vol.16, Issue 1, 2010
Vol.15, Issue 04, 2009
Vol.15, Issue 03, 2009
Vol.15, Issue 02, 2009
Vol.15, Issue 1, 2009
Vol.14, Issue 04, 2008
Vol.14, Issue 2-3, 2008
Vol.14, Issue 2-3, 2008
Vol.14, Issue 1, 2008
Vol.14, Issue 2-3, 2008
Vol.13, Issue 04, 2007
Vol.13, Issue 2, 2007
Vol.13, Issue 1, 2007
Vol.12, Issue 4, 2006
Vol.12, Issue 3, 2006
Vol.12, Issue 2, 2006
Vol.12, Issue 1, 2006
Vol.12, Issue 01, 2006
Vol.11, Issue 3,4, 2005
Vol.11, Issue 2, 2005
Vol.11, Issue 1, 2005
Vol.10, Issue 04, 2004
Vol.10, Issue 03, 2004
Vol.10, Issue 02, 2004
Vol.10, Issue 01, 2004
Vol.09, Issue 04, 2003
Vol.09, Issue 03, 2003
Vol.09, Issue 02, 2003
Vol.08, Issue 04, 2002
Vol.08, Issue 03, 2002
Vol.08, Issue 01, 2002
Vol.07, Issue 04, 2001
Vol.07, Issue 03, 2001
Vol.07, Issue 02, 2001
Vol.07, Issue 01, 2001
Vol.06, Issue 04, 2000
Vol.06, Issue 03, 2000
Vol.06, Issue 02, 2000
Vol.06, Issue 01, 2000
Vol.05, Issue 04, 1999
Vol.05, Issue 03, 1999
Vol.05, Issue 02, 1999
Vol.05, Issue 01, 1999
Vol.04, Issue 1,2, 1998
Vol.03, Issue 3,4, 1997
Vol.03, Issue 01, 1997
Vol.02, Issue 1,2, 1996
Vol.01, Issue 14, 1995
Looking for Past Issues? Click here to get them!!