Ecology, Environment and Conservation Paper

BIOCHEMICAL SCREENING OF AMYLOSE CONTENT IN GRAIN OF PROSO MILLET (PANICUM MILIACEUM L.) COLLECTION

Rysbekova Aiman Bokenovna Rysbekova, Dyussibayeva Elmira Navrusbekovna, Zhirnova Irina Alexandrovna, Seitkhozhaev Abilbashar Ilyasovich, Zhakenova Aiym Erbolkyzy and Vladimir Igorevich Tsygankov

Abstract

In the present study, we conducted the quantitative amylose content in grain of proso millet collection from different ecological-geographical origin (Afghanistan, Australia, Belarus, Canada, China, France, Hungary, India, Iran, Kazakhstan, Kyrgyzstan, Pakistan, Russian Federation, Tajikistan, Tatarstan, Turkey, Ukraine, USA and Uzbekistan). Amylose content was determined using AACCI method by an iodine-staining (I2- KI) assay. Generally, 197 genotypes were analysed and their conditional classification were composed. According the conditional classification, it was revealed that the amylose content was very high in 19.29% of the studied samples, 44.67% were high amylose groups, 20.81% were belong to intermediate groups, 12.69% were low amylose groups and only 2.54% were waxy groups (Figure 2). The results are shown that only five genotypes belong to the waxy type: K-10343, K-3742, PI 436626 (Lung Shu 18), PI 436625 (Lung Shu 16) and Ma zha Yan. Selected genotypes represent valuable genetic material for the creation of glutinous proso millet varieties and will be involved in the breeding programme.