Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Nov. Suppl. Issue, 2019; Page No.(116-121)

ISOLATION AND MOLECULAR CHARACTERIZATION OF BRUCELLA MELITENSIS FROM DAIRY PRODUCTS OF GOATS AND SHEEP IN RAPARIN DISTRICT, KURDISTAN REGION, IRAQ

RZGAR Q. PIROT AND ADEL K. KHDR

Abstract

Brucellosis is a zoonotic disease that is found in animals and humans all over the world which is caused by Brucellaspecies. In this study which was performed to isolation and identification Brucellamelitensis from dairy products samples collected from some herds in Raparin district. In this study Serological test such as RBPT and 2ME test was used. Cultivation of bacteria was performed on some selective and deferential media such as Brain heart infusion broth, Brucella broth, Brucella agar, Colambiaagar and farallel selective medium, Isolation by using some biochemical tests such: (Oxidase, catalase,urease, blood hemolysis, and CO2 requirement) were done. A Polymerase Chain Reaction (PCR) was used for identification of Brucellaspp through detection bscp31 and IS711 genes from isolated bacteria. 130 positive of Rose Bengal Test were obtained which was performed previously on 520 blood samples that were collected from 36 herds of goats and sheep in this area. Among 144 total dairy product samples 14 isolates of Brucellamelitensis were cultured. Ten isolates from raw milk, 4 isolates from cheese samples and 2 isolates from human blood. For supporting the identification process, a molecular technique as confirmatory method through detection bscp31 and IS711 genes were performed. The prevalence of Brucellosis in livestock animals(sheep and goats) and human in Raparin district is endemic at significant rates and should be considered as an important health problem in this area.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 22, Issue 2, 2020
Vol 22, Issue 1, 2020
Vol 21, Issue 4, 2019
Vol 21, Nov. Suppl. Issue, 2019
Vol 21, Issue 3, 2019
Vol 21, Issue 2, 2019
Vol 21, Issue 1, 2019
Vol 20, Dec. Suppl. No.2, 2018
Vol 20, Issue 4, 2018
Vol 20, Dec. Suppl. Issue, 2018
Vol 20, Oct Suppl. Issue, 2018
Vol 20, Issue 3, 2018
Vol 20, Issue 2, 2018
Vol 20, Issue 1, 2018
Vol 20, Feb. Suppl. Issue, 2018
Vol 19, Issue 4, 2017
Vol 19, Nov. Suppl, Issue 2017
Vol 19, Issue 3, 2017
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!