Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Nov. Suppl. Issue, 2019; Page No.(84-90)

ISOLATION AND CHARACTERIZATION OF ARSENIC RESISTANT BACTERIA FROM GROUNDWATER FROM LAKHIMPUR DISTRICT

B.K. PEGU, M. BURAGOHAIN, N. KAKOTI, P. SARMAH, S.R. MAHANTA AND M. SHARMA2

Abstract

The aim of this study was to isolation and characterization of the arsenic (As) resistant bacteria from arsenic contamination sites of Lakhimpur districts, Assam, India. This investigation, estimation of arsenic content from water samples are found compared to higher concentrations (0.26±0.04 mg/L) of arsenic than the permissible limit of the World Health Organization (WHO). In total 10th morphologically completely different arsenic- resistant microorganism isolated from water samples in, among one them GB-18, the very best resistant to others. This potential isolate identified by morphological, biochemical and molecular characterization the bacteria GB-18 was a Pseudomonas aeruginosa. The bacterial growth kinetics of strain GB-18 were changed slightly in the presence of 5mM As (V) and 1mM As (III) stress compared tocontrol. The bacterium was high tolerance to the above concentration of 20mM As (V) and 5mM of As (III).In this study major fatty acids found were, 16:0 (18.31%), 10:03OH (8.52%), 12:02OH (8.52%), 12:0 (5.24%).The GB-18 optimum growth of pH and temperature were observed 6.0 and 30 oC. The bacterium strain Pseudomonas aeruginosa–GB18 can survive below the high concentration of arsenic stress and results prompt that a possible for removal of arsenic in contaminated environments.

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