Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Nov. Suppl. Issue, 2019; Page No.(17-25)

HALOTOLERANT AND THERMOSTABLE PROTEASES FROM BACILLUS SPP. ISOLATED FROM THE MANGROVE FOREST

PIJITRA SAELAOA, DUANGJAI SITTIPOLA, MANCHITA MAHAWONGA, TITA FOOPHOWB, JANPEN TANGJITJAROENKUNC AND NUJARIN JONGRUJAA

Abstract

Mangrove ecosystem is one of the tropical ecosystems that have rich biological resources. As the coastal saline is in the ecosystem, microorganisms founded in the system usually show a halophilic orhalotolerant characteristic which is beneficial to the industrial applications. In this research, we isolated microorganisms from the site. It has been shown that five bacterial isolates from the mangrove eco-system exhibited proteolytic activity on the skimmed milk agar containing salt. The bacterial isolates have been analyzed 16S rRNA genes and indicated that they are Bacillus spp. The productions of the protease from each isolate suggest that the productions of proteases reach the maximal level at 12-hour incubation at 37oC. The optimum conditions for enzymatic activity have been examined indicating that the proteases of isolates show a variation of optimum conditions. Among them, the isolate TS21 has shown the high enzymaticactivity and performed the maximum activity at 60°C, pH 10 in the presence of 1.0 M - 1.5 M NaCl. The protease from KB16 has shown different optimum conditions which are 60oC, neutral pH in the presence of 1.0 M NaCl. The proteases of other isolates also have different conditions for their high enzymatic activities suggesting various applications for each of them.

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