Pollution Research Paper

DOSE-RESPONSE ANALYSIS OF FERTERRA 0.4% GR ON HETEROPNEUSTES FOSSILIS (BLOCH) THROUGH HISTOPATHOLOGICAL MANIFESTATIONS: A COMPARATIVE ANALYSIS

ARNAB KUMAR MONDAL AND APURBA RATAN GHOSH

Abstract

Histopathological lesions in liver, kidney, stomach and intestine of freshwater fish, Heteropneustes fossilis (Bolch) under the exposure of three different doses 10% (0.044 g, i.e., T1), 20% (0.088 g i.e., T2) and 30% (0.132 g i.e., T3) of LC50 value (11.008 mg/L) of Ferterra 0.4% GR (Chlorantraniliprole) for 15 days were compared through Haematoxylin-Eosin staining method under light microscopy. Degeneration of hepatic cords of hepatic lobules and distortion of acinar cells were prominent at T2 and T3 levels than at T1 level; hepatopancreas showed serious damage at T3 level. In kidney, proximal and distal convoluted tubules and collecting ducts revealed deformation at T2 level, but degree of destruction and shrinkage of glomeruli were more prominent at T3 level; in addition, DCT and PCT displayed more degeneration along with haemopoietic tissue at T3 level. In stomach, shrinkage of mucosal folds, vacuolization of mucosa and submucosa, distortion of lamina propria at T2 level were significant. In intestine, damage of CEC and mucosal folds were not so prominent as compared to T2 and T3, where the degree of changes in the basement membranes, fusion of intestinal villi, lesions in brush borders and CEC were very significant at T2 and at T3 levels. This study compared the degree of changes due to Ferterra intoxication at different doses, and inferred that the dose of Ferterra as insecticide must be monitored considering the health risk of human beings as the consumer of fish, the most important aquatic protein source.