OPTIMIZATION OF BIOSURFACTANT PRODUCTION FROM B. LICHENIFORMIS AND STUDIES ON ITS ANTI-ADHESIVE PROPERTYM. J. Rathod, V. B. Gaikwad and S.N. PatilAbstract Biosurfactants are a diverse group of surface active molecules exhibiting wide applications. The present study was done to isolate biosurfactant producing bacteria from different samples which included oil contaminated sludge (Mazgaon dock, Mumbai), garage and generator soil mixed with petrochemicals and oil contamimated marine water (Uran, Mumbai). 75 isolates were initially screened by blood hemolysis, emulsification index and oil displacement test. 3 out of 75 isolates showed good biosurfactant activity in primary and secondary screening. Isolate GO2 identified as Bacillus licheniformis showed a reduction in surface tension of water to 32 mN /m. The biosurfactant production by B. licheniformis was found to be maximum in presence of groundnut and grapeseed oil and using tri-ammonium citrate as nitrogen source. The optimum conditions of pH, temperature, salt concentration and inoculum size were 7, 40 oC, 0.5% and 3 % respectively. The biosurfactant was characterized as lipopeptide in nature. The media optimization for biosurfactant productivity yielded 3.8 g/L biosurfactant. It showed anti-adhesive activity against Biofilm forming bacteria isolated from contact lens samples. The maximum biofilm inhibition activity for P. aeurginosa, S. epidermidis, S.aureus and K. pneumoniae was found to be 70.5%, 68.43%, 66%, 62.62% and respectively. Thus a potential biosurfactant with good anti-adhesive activity has been isolated and optimized for its production and further application.
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